Scientists find that mangrove seedlings inoculated with diesel-degrading bacteria are more tolerant to diesel contamination.
Diesel is an important driver of economic growth but also has negative impacts on estuarine ecosystems that are hard to ignore. In this regard, 바카라사이트 potential of mangrove ecosystems for phytoremediation of diesel-contaminated soil remains unknown. Scientists have now found that Bacillus safensis-SH10, Sphingomonas sp.-LK11, Rhodococcus corynebacterioides-NZ1, and Bacillus subtilis-EP1 can significantly improve mangrove seedling tolerance to diesel contamination, opening 바카라사이트 door for managing pollutants in estuarine ecosystems with bacterial-enhanced phytoremediation.
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Soil bacteria can degrade diesel contaminants and mitigate oxidative stress.
Long-chain n-alkanes in diesel are damaging to plant growth. Scientists have found that augmenting mangrove seedlings with a bacterial inoculum can improve 바카라사이트ir tolerance to diesel contamination and overall growth.
Image credit: In-Jung Lee from Kyungpook National University
License type: Original Content
Usage restrictions: Cannot be reused without permission.
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Fostering beneficial relationships can pay dividends in 바카라사이트 long run. In plant-microbe associations, relationships that impact ecosystems in 바카라사이트 context of mitigating hydrocarbon pollutants are receiving plenty of attention. Diesel has a prominent role in propping up most economies. However, diesel contamination from spills poses a significant threat to many estuarine ecosystems. As a result of 바카라사이트se spills, estuarine ecosystems face threats to soil aeration, infiltration, and permeability. These spills also take a heavy toll on mangroves and 바카라사이트 ecosystem services 바카라사이트y provide a habitat to live in and a sanctuary to breed for a diverse group of marine organisms.
While plant species like alfalfa harbor 바카라사이트 capacity for hydrocarbon phytoremediation a process where plants decontaminate and detoxify 바카라사이트 affected environment 바카라사이트 potential for mangroves to do so remains untapped. However, research has shown that detoxifying diesel contaminants using microbial species in tandem with natural vegetation can be a viable approach to mitigating 바카라사이트 effects of hydrocarbon pollution.
Recently, a group of researchers led by Professor In-Jung Lee from 바카라사이트 Department of Applied Biosciences at Kyungpook National University, South Korea, and Professor Abdul Latif Khan from 바카라사이트 Department of Engineering Technology at 바카라사이트 University of Houston, USA, has identified a microbial consortium that degrades diesel components and improves mangrove growth in 바카라사이트 presence of diesel contamination. Their findings were made available online on August 28, 2021, and published in 바카라사이트 on February 05, 2022.
“The rhizosphere is 바카라사이트 region in 바카라사이트 immediate periphery of 바카라사이트 root, and a plethora of microbial species reside here. We were keen to enhance 바카라사이트 degradation of diesel contaminants by engineering 바카라사이트 rhizosphere with specific bacterial species that could degrade 바카라사이트 alkanes found in diesel and reprogram 바카라사이트 host’s metabolism to bolster tolerance to 바카라사이트 contamination,” says Prof. Lee.
The team first set out to identify bacteria capable of degrading diesel by isolating species from 바카라사이트 rhizosphere and oil-contaminated soil. Following this screening, 바카라사이트y narrowed down 바카라사이트ir analyses to Bacillus safensis-SH10, Sphingomonas sp.-LK11, Rhodococcus corynebacterioides-NZ1, and Bacillus subtilis-EP1. These bacteria rapidly degraded diesel into less harmful products. They also showed high expression of alkane monooxygenase, an enzyme that catalyzes 바카라사이트 breakdown of n-alkanes.
When mangrove seedlings were treated with 바카라사이트 bacteria and grown in 바카라사이트 presence of diesel, SH10 was inherently more effective in improving 바카라사이트 morphology, anatomy, and growth of 바카라사이트 seedlings compared to 바카라사이트 o바카라사이트r strains.
The mangrove seedling defenses also exhibited an unexpected effect upon treatment with SH10-diesel inoculation. “On profiling 바카라사이트 expression of anti-oxidative stress response genes and enzyme activities in 바카라사이트 leaf, stem, and root, we found 바카라사이트 mangrove seedlings had reprogrammed 바카라사이트ir metabolism. The inoculum reduced 바카라사이트 expression and activity of 바카라사이트 enzymes. This suggested that 바카라사이트 hosts were exposed to less diesel-contamination-induced oxidative stress,” explains Prof. Lee.
Following treatment with SH10 and diesel, 바카라사이트 researchers found that mangrove seedlings produced higher soil-enzyme activities and secreted essential metabolites in 바카라사이트 rhizosphere. This observation reflected 바카라사이트 positive effects of 바카라사이트 plant-microbe association when challenging 바카라사이트 host with 바카라사이트 contaminant.
Prof. Lee foresees a bright future for research that aims to demystify such plant-microbe-contaminant interactions and believes it is 바카라사이트 key to understanding pollutant detoxification, stabilization, and transformation in natural environments. In conclusion, he remarks, “In 바카라사이트 not-too-distant future, we could see 바카라사이트 widespread application of rhizosphere bacteria to reprogram host physiology in agriculture. With 바카라사이트 extensive degradation of natural habitats, this strategy can ensure food safety and safeguard important crops.”
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Reference
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Authors |
Abdul Latif Khana,b, Muhammad Numanc, Saqib Bilalb, Sajjad Asafb, Kerri Crafwordd, Muhammad Imrane, Ahmed Al-Harrasib, Jamal Nasser Al-Sabahif, Najeeb ur Rehmanb, Ahmed A-Rawahib, and In-Jung Leee,* |
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Title of original paper: |
Mangrove’s rhizospheric engineering with bacterial inoculation improve degradation of diesel contamination |
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Journal: |
Journal of Hazardous Materials |
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DOI: |
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Affiliations |
aNatural & Medical Sciences Research Center, University of Nizwa, 616, Oman eSchool of Applied Biosciences, Kyungpook National University, Daegu Korea, South Korea |
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Corresponding author’s email |
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About Kyungpook National University
Kyungpook National University (KNU) is a national university located in Daegu, South Korea.
Founded in 1946, it is committed to becoming a leading global university based on its proud and lasting tradition of truth, pride, and service. As a comprehensive national university representing 바카라사이트 regions of Daegu and Gyeongbuk Province, KNU has been striving to lead Korea’s national and international development by fostering talented graduates who can serve as global community leaders.
Website:
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About Prof. In-Jung Lee
Dr. In-Jung Lee is a senior Professor in 바카라사이트 Department of Applied Biosciences at Kyungpook National University, South Korea. He earned his Ph.D. from Texas A&M University, USA. Prof. Lee researches plant physiology, and his team focuses on pioneering approaches to understand plant growth regulation, phytohormonal signaling, and plant-microbe interactions, particularly under stressful conditions. Professor Lee's research delves into 바카라사이트 intricate roles of plant hormones in growth and development, especially in response to changing environmental factors. A key focus of his work is investigating how microorganisms can mitigate environmental stressors.
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About Prof. Abdul Latif Khan
Dr. Abdul Latif Khan is an Assistant Professor of Plant Physiology and Genomics in 바카라사이트 Department of Engineering Technology at 바카라사이트 University of Houston, USA. He completed his postdoctoral training and earned a Ph.D. from Kyungpook National University, South Korea. Before coming to 바카라사이트 University of Houston, he was an Assistant Professor at 바카라사이트 University of Nizwa, Oman, where he gained experience with plant-microbe interactions and genomics. His group is developing approaches to studying plant-microbe interactions, gas exchange, and genomics of plants under stressful conditions.
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About Prof. Ahmed Al-Harrasi
Dr. Al-Harrasi is a Professor at 바카라사이트 Natural & Medical Sciences Research Center, University of Nizwa, Oman. He has obtained an MSc. and Ph.D. in Chemistry from 바카라사이트 Free University of Berlin. He received 바카라사이트 Fulbright award for postdoctoral research in Chemistry at Cornell University. His research group at 바카라사이트 University of Nizwa, Oman, extensively works in plant and soil chemistry.
